Other

Part:BBa_K3252042:Design

Designed by: Alfin Mohammad Abdillah   Group: iGEM19_ITB_Indonesia   (2019-10-21)


LuxOUN-gRNA Actuator Module


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6080
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4254
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 853
    Illegal SapI site found at 2457


Design Notes

This part was assembled with Golden Gate and BioBricks assembly method using BsaI Type IIS restriction enzyme and EcoRI-XbaI enzymes.


Source

This part sequence was adapted from this reference.

Holowko, M. B., Wang, H., Jayaraman, P., Poh, C. L., (2016). Biosensing Vibrio cholerae with Genetically Engineered Escherichia coli. ACS Synth. Biol. 2016, 5, 1275-1283.

References