Other
Part:BBa_K3252042:Design
Designed by: Alfin Mohammad Abdillah Group: iGEM19_ITB_Indonesia (2019-10-21)
LuxOUN-gRNA Actuator Module
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 6080
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4254
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 853
Illegal SapI site found at 2457
Design Notes
This part was assembled with Golden Gate and BioBricks assembly method using BsaI Type IIS restriction enzyme and EcoRI-XbaI enzymes.
Source
This part sequence was adapted from this reference.
Holowko, M. B., Wang, H., Jayaraman, P., Poh, C. L., (2016). Biosensing Vibrio cholerae with Genetically Engineered Escherichia coli. ACS Synth. Biol. 2016, 5, 1275-1283.